Barley germination cell-specific transcriptomes: P48h

Sample Details

Plumule, 48-hour

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Data-set Details

We used laser capture microdissection to precisely isolate cells from three embryo organs (plumule, radicle tip, scutellum) over a time course during germination (0-48 h, 7 time points) then analyzed their transcriptomes using RNA-sequencing. These organs have distinct functions as the embryo transitions from germinating seed to seedling. These analyses revealed that the spatiotemporal pattern of defined cells different to bulk analyses, that there is cell-specific storage of transcripts during seed development and embryo maturation, there were 16 distinct patterns of gene expression, with the majority (14 of 16) specific to one organ and a subset of timepoints during germination, rather than being shared between organs and gene regulatory network analyses identified known and novel regulatory processes.