Barley germination cell-specific transcriptomes

We used laser capture microdissection to precisely isolate cells from three embryo organs (plumule, radicle tip, scutellum) over a time course during germination (0-48 h, 7 time points) then analyzed their transcriptomes using RNA-sequencing. These organs have distinct functions as the embryo transitions from germinating seed to seedling. These analyses revealed that the spatiotemporal pattern of defined cells different to bulk analyses, that there is cell-specific storage of transcripts during seed development and embryo maturation, there were 16 distinct patterns of gene expression, with the majority (14 of 16) specific to one organ and a subset of timepoints during germination, rather than being shared between organs and gene regulatory network analyses identified known and novel regulatory processes.

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Samples

P0h

Plumule, 0-hour

P8h

Plumule, 8-hour

P16h

Plumule, 16-hour

P24h

Plumule, 24-hour

P32h

Plumule, 32-hour

P40h

Plumule, 40-hour

P48h

Plumule, 48-hour

R0h

Radicle, 0-hour

R8h

Radicle, 8-hour

R16h

Radicle, 16-hour

R24h

Radicle, 24-hour

R32h

Radicle, 32-hour

R40h

Radicle, 40-hour

R48h

Radicle, 48-hour

S0h

Scutellum, 0-hour

S8h

Scutellum, 8-hour

S16h

Scutellum, 16-hour

S24h

Scutellum, 24-hour

S32h

Scutellum, 32-hour

S40h

Scutellum, 40-hour

S48h

Scutellum, 48-hour